Protein Concentration SOP: Bradford Assay

Shalaka Samant, PhD • January 21, 2020
Protein Concentration SOP: Bradford Assay

SOP: Protein Reagent Concentration

Determining Sample Consistency

Whether your lab already has an SOP in place for new protein analysis or would benefit from a revised procedure, we provide a simplified version of kbDNAs in-house Bradford Assay protocol for concentration testing commercial protein reagents.


Why concentration and why now?

> LAB REPLENISHING

Many researchers have begun ordering recombinant reagents as part of their material replenishing & it's important to revisit the role of concentration

> COVID IMPACT

Due to the unique disruption in the manufacturing sector, the likelihood of receiving proteins from the same batch (or lot) prior to COVID is low

> CONCENTRATION FACTOR

Measuring sample concentration and comparing it to the data on the certificate of analysis is the most direct way to determine whether any quality was impacted

> VERIFYING QUALITY

We suggest running Bradford assay tests on both same and different lot samples since the disruption could have occurred during the production or storage period

JPM 52:25

By Ed Hamdeh • January 7, 2025
Explore key insights and major trends from a decade of JPM Biotech conferences. Download our exclusive 2025 prospectus for the latest in biotech deals and innovations.
The Advantages and Applications of Recombinant technology | kbDNA

Advantages and Applications of Recombinant Antibodies

By Laith Khalil • October 25, 2024
Explore the advantages of recombinant antibodies. Discover their applications in research, diagnostics & therapeutics. Transform your R&D with precision.
kbDNA | Specialty Synthesis & Recombinant Technology

FLAG Tag: A Comprehensive Overview for Biopharmaceutical Research

By kBDNA Team • October 25, 2024
The FLAG tag (DYKDDDDK) is an epitope tag widely utilized in biopharmaceutical research for protein purification, detection, and functional analysis. This article provides an in-depth examination of FLAG tag technology, its advantages, limitations, and diverse applications. Additionally, it explores current research trends and future directions in FLAG tag technology, making it a pivotal tool in advancing drug discovery and development.