Nucleotide Synthesis

DNA & RNA oligos with high modification + length capacity

Quality Control Criteria

UV Characterization

Thin Layer Chromatography

Coupling Test

HPLC

Solubility Test

Nucleotide Synthesis Technology



Harnessing building blocks to develop natures tools. kbDNA’s platform is powered by diverse technical experience in nucleic acid chemistry. This has allowed the integration of proprietary reagents and over 40 years of efficient click-chemistry synthesis to operate under one roof and produce novel ssDNA and RNA.

Our focus is to continue optimizing automated synthesis. This is achieved by chemical intervention at specific solid-phase production points. Thereby reinforcing each step with specialty support to perform target reaction. As a result, kbDNA enables researchers with short (oligo) and long nucleotide sequences to meet their experimental design.


Upgrading Automation in Solid-Phase Synthesis 

01

Detritylation

Step 1 requires starting with a support monomer - traditionally with Controlled Pore Glass (CPG) attached.

Upgrading Automation in Solid-Phase Synthesis 

02

Coupling

Step 2 involves the coupling of the next monomer with the previous monomer or first support

03

Oxidation

Step 3 follows with eliminating the presence of failure sequences by attaching blocking groups to unreacted sites- improving synthesis fidelity

04

Capping

Stabilizing the phosphate backbone allows for the introduction of the next monomer through continuous repeat of the cycle until the desired sequence is achieved.

05

Completion

Cleavage of all protecting groups off the final product. Cleavage of the final product yields biologically active molecule.

The kbDNA Advantage

Speed

Custimization

Validation

Expertise

Control Your Parameters

Lengths

With our platform, you’ll save time when you need it most. It’s the fastest way to meet your customers’ needs

Modifications

Streamline Catapult your growth by streamlining services and features.

Purity

Our support team is available every day to make sure that things runs smoothly. We’re here to help. 

Scale-Up

You’ll enjoy company growth faster than you expected possible, by streamlining services and features. 

Applications

PCR | qPCR

Gene Editing | CRISPR

Antisense | siRNA

miRNA | circRNA

LNP's | Therapy

How to Order from kbDNA

STREAMLINED ORDER PROCESS

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    Step 1: Analyze

    Input your sequence(s) in our calculator (SeqSCREEN)

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    Step 2: Submit

    Paste your calculator results into the submission form below the calculator and click submit.

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    Step 3: Place Your Order

    You'll get pricing and lead time by email within 2 hours.  Follow the link to complete your order!

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Get Started Today

Start New Project

Our tool is designed with scientists in mind.


It simplifies the process of designing a nucleotide synthesis project by utilizing a user friendly interface in a quick and easy four step process.

Submit Existing Project

Already have nucleotide sequences ready to go?


You can paste or upload them directly along with any notes or questions.

We'll proofread them and contact you within a day!

Start New Project

Our tool is designed with scientists in mind.


It simplifies the process of designing a nucleotide synthesis project by utilizing a user friendly interface in a quick and easy four step process.

Learn More

Submit Existing Project

Already have nucleotide sequences ready to go?


You can paste or upload them directly along with any notes or questions.


We'll proofread them and contact you within a day!

Learn More

Nucleotide Synthesis Technology

Once you submit your inquiry - we'll review your submission and reply within 24 hours. Our response will include:


  • The results of our free proofread, conducted by our expert chem-team.
  • Recommendations for enhancing your oligos' performance based on your requirements.
  • Quotes for both your original project submission, and a project utilizing our recommendations.

The Ultimate Guide for Building Oligos

The Reagent Table of Nucleotides
The periodic table of elements

Manufacturing is in the Genes

Experience and credibility play significant roles in bio scientific research. ChemGenes reagents have been tried and true for decades, contributing non-stop innovations to improve coupling efficiency and the structural capabilities of automated synthesis. Their proven quality and expertise make them not only the most qualified, but also the best partner to compliment kbDNA’s synthesis platform..

Patents & Trademarks
  • 2’-O-Methyl, Ethyl phosphoramidite, patent #: US 6,015,886.
  • 2’-O-Methyl patent, patent #: US 5,525,719.
  • 3’-O-Methyl patent #: US 5,525,719.
  • 2’-O-Propargyl modified, patent #: US 5,744,595.
  • 3’-O-Propargyl modified Amidites & Solid Supports, patent #: US 5,744,595.
  • 2’-Ara-O-methyl amidites, Patent #: US 20120149888.
  • Reverse RNA synthesis, patent App #: WO 2011103468.
  • Dithiolane based thiol modifier, patent Provisional application #: PCT61/79585.
  • Oligonucleotide Labeling-Cycloaddition Reaction, Patent #: US 20120058476.
  • Modifications of antimetabolite gemcitabine for incorporation in CpG oligonucleotides, Patent #: US 20110040082
  • N6-Trimethyl-L-Lysine & derivative, their therapeutic uses, Patant #: US 20110166230; US 20110166231; US 20110166379; US 20110166380