Cystatin D

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

16080

Length (aa):

142

Sequence:

MMWPMHTPLLLLTALMVAVAGSASAQSRTLAGGIHATDLNDKSVQCALDFAISEYNKVINKDEYYSRPLQVMAAYQQIVGGVNYYFNVKFGRTTCTKSQPNLDNCPFNDQPKLKEEEFCSFQINEVPWEDKISILNYKCRKV

Proteomics (Proteome ID):

Cystatin-D (Cystatin-5)

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

Mass=13154.4675; Mass_error=0.0101; Method=Electrospray; Range=29-142; Note=Variant Arg-46.; Evidence={ECO:0000269|PubMed:20189825}; Mass=13596.7015; Mass_error=0.0049; Method=Electrospray; Range=25-142; Note=Variant Arg-46.; Evidence={ECO:0000269|PubMed:20189825};

Function [CC]:

Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. {ECO:0000269|PubMed:8083219}.

Metal Binding:

N/A

Site:

SITE 32 32 Reactive site. {ECO:0000250}.

Tissue Specificity:

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall bladder tissue. {ECO:0000269|PubMed:20189825}.

Disease:

N/A

Mutagenesis:

N/A

Name:

Cystatin-D (Cystatin-5)

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.