Hemagglutinin

Gene Synonyms:

Protein Names:

Organism:

Influenza A virus (A/Shanghai/02/2013(H7N9))

Mass (kDa):

62108

Length (aa):

560

Sequence:

MNTQILVFALIAIIPTNADKICLGHHAVSNGTKVNTLTERGVEVVNATETVERTNIPRICSKGKRTVDLGQCGLLGTITGPPQCDQFLEFSADLIIERREGSDVCYPGKFVNEEALRQILRESGGIDKEAMGFTYSGIRTNGATSACRRSGSSFYAEMKWLLSNTDNAAFPQMTKSYKNTRKSPALIVWGIHHSVSTAEQTKLYGSGNKLVTVGSSNYQQSFVPSPGARPQVNGLSGRIDFHWLMLNPNDTVTFSFNGAFIAPDRASFLRGKSMGIQSGVQVDANCEGDCYHSGGTIISNLPFQNIDSRAVGKCPRYVKQRSLLLATGMKNVPEIPKGRGLFGAIAGFIENGWEGLIDGWYGFRHQNAQGEGTAADYKSTQSAIDQITGKLNRLIEKTNQQFELIDNEFNEVEKQIGNVINWTRDSITEVWSYNAELLVAMENQHTIDLADSEMDKLYERVKRQLRENAEEDGTGCFEIFHKCDDDCMASIRNNTYDHSKYREEAMQNRIQIDPVKLSSGYKDVILWFSFGASCFILLAIVMGLVFICVKNGNMRCTICI

Proteomics (Proteome ID):

Hemagglutinin [Cleaved into: Hemagglutinin HA2 chain; Hemagglutinin HA1 chain]

Proteomics (Chromosome):

UP000135035

Mass Spectrometry:

N/A

Function [CC]:

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. {ECO:0000256|HAMAP-Rule:MF_04072, ECO:0000256|SAAS:SAAS01039073}.; Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. {ECO:0000256|RuleBase:RU003324}.

Metal Binding:

METAL 89 89 Calcium. {ECO:0000213|PDB:4LN6, ECO:0000213|PDB:4LN8}.; METAL 127 127 Calcium. {ECO:0000213|PDB:4LN6, ECO:0000213|PDB:4LN8}.; METAL 128 128 Calcium; via carbonyl oxygen. {ECO:0000213|PDB:4LN6, ECO:0000213|PDB:4LN8}.

Site:

SITE 339 340 Cleavage; by host. {ECO:0000256|HAMAP-Rule:MF_04072}.

Tissue Specificity:

N/A

Disease:

N/A

Mutagenesis:

N/A

Name:

Hemagglutinin [Cleaved into: Hemagglutinin HA2 chain; Hemagglutinin HA1 chain]

Class:

Subcategory:

Recombinant

Source:

Species:

Influenza

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.