LAG-3

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

57449

Length (aa):

525

Sequence:

MWEAQFLGLLFLQPLWVAPVKPLQPGAEVPVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSCRLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPHHHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRSFSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHLLLFLILGVLSLLLLVTGAFGFHLWRRQWRPRRFSALEQGIHPPQAQSKIEELEQEPEPEPEPEPEPEPEPEPEQL

Proteomics (Proteome ID):

Lymphocyte activation gene 3 protein (LAG-3) (CD antigen CD223) [Cleaved into: Secreted lymphocyte activation gene 3 protein (sLAG-3)]

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

N/A

Function [CC]:

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:7805750, PubMed:8647185, PubMed:20421648). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185). {ECO:0000250|UniProtKB:Q61790, ECO:0000269|PubMed:20421648, ECO:0000269|PubMed:7805750, ECO:0000269|PubMed:8647185}.; Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. {ECO:0000250|UniProtKB:Q61790}.

Metal Binding:

N/A

Site:

N/A

Tissue Specificity:

Primarily expressed in activated T-cells and a subset of natural killer (NK) cells. {ECO:0000269|PubMed:1380059, ECO:0000269|PubMed:1692078, ECO:0000269|PubMed:20421648}.

Disease:

N/A

Mutagenesis:

MUTAGEN 35 35 Q->A: Does not affect binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 52 52 D->A: Reduced binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 78 78 H->A: Reduced binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 78 78 H->F: Does not significantly affect binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 85 85 H->A,F: Does not affect binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 95 95 R->E: Increased binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 97 97 R->A,E: Increased binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 98 98 R->E: Increased binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 99 99 Y->F: Abolishes binding to MHC class II (MHC-II) without affecting interaction with FGL1. {ECO:0000269|PubMed:30580966, ECO:0000269|PubMed:9159144}.; MUTAGEN 110 110 R->A: Reduced binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 125 125 R->A: Reduced binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 129 129 R->K: Does not affect binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 131 131 D->A: Reduced binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 137 137 R->A: Reduced binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 155 156 DR->AA: Slightly affects binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.; MUTAGEN 247 247 D->L: Does not affect binding to MHC class II (MHC-II). {ECO:0000269|PubMed:9159144}.

Name:

Lymphocyte activation gene 3 protein (LAG-3) (CD antigen CD223) [Cleaved into: Secreted lymphocyte activation gene 3 protein (sLAG-3)]

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.