Annexin A1

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

38714

Length (aa):

346

Sequence:

MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

Proteomics (Proteome ID):

Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35)

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

N/A

Function [CC]:

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). {ECO:0000250|UniProtKB:P10107, ECO:0000250|UniProtKB:P19619, ECO:0000269|PubMed:15187149, ECO:0000269|PubMed:17008549, ECO:0000269|PubMed:19625660, ECO:0000269|PubMed:2532504, ECO:0000269|PubMed:25664854, ECO:0000269|PubMed:2936963, ECO:0000269|PubMed:8425544, ECO:0000269|PubMed:8557678}.

Metal Binding:

METAL 59 59 Calcium 1; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 60 60 Calcium 1; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 62 62 Calcium 1. {ECO:0000250|UniProtKB:P19619}.; METAL 97 97 Calcium 2; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 100 100 Calcium 2; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 105 105 Calcium 2. {ECO:0000250|UniProtKB:P19619}.; METAL 127 127 Calcium 3; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 129 129 Calcium 3; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 131 131 Calcium 3; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 132 132 Calcium 4; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 134 134 Calcium 4. {ECO:0000250|UniProtKB:P19619}.; METAL 171 171 Calcium 3. {ECO:0000250|UniProtKB:P19619}.; METAL 210 210 Calcium 5; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 213 213 Calcium 5; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 215 215 Calcium 5; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 253 253 Calcium 6. {ECO:0000250|UniProtKB:P19619}.; METAL 255 255 Calcium 5. {ECO:0000250|UniProtKB:P19619}.; METAL 256 256 Calcium 6; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 261 261 Calcium 6. {ECO:0000250|UniProtKB:P19619}.; METAL 286 286 Calcium 7; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 288 288 Calcium 7; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 290 290 Calcium 7; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 328 328 Calcium 8; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 330 330 Calcium 7. {ECO:0000250|UniProtKB:P19619}.; METAL 331 331 Calcium 8; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 336 336 Calcium 8. {ECO:0000250|UniProtKB:P19619}.

Site:

N/A

Tissue Specificity:

Detected in resting neutrophils (PubMed:10772777). Detected in peripheral blood T-cells (PubMed:17008549). Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:25664854). Detected in placenta (at protein level) (PubMed:2532504). Detected in liver. {ECO:0000269|PubMed:2532504, ECO:0000269|PubMed:2936963}.

Disease:

N/A

Mutagenesis:

MUTAGEN 27 27 S->A: Abolishes secretion and modulation of exocytosis. {ECO:0000269|PubMed:19625660}.; MUTAGEN 34 34 S->A: No effect on secretion and modulation of exocytosis. {ECO:0000269|PubMed:19625660}.; MUTAGEN 45 45 S->A: Abolishes secretion and nearly abolishes modulation of exocytosis. {ECO:0000269|PubMed:19625660}.

Name:

Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35)

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Solution

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 95%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

Inquire

Storage:

4°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.