ENPP-2

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

98994

Length (aa):

863

Sequence:

MARRSSFQSCQIISLFTFAVGVNICLGFTAHRIKRAEGWEEGPPTVLSDSPWTNISGSCKGRCFELQEAGPPDCRCDNLCKSYTSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLARGDCCTNYQVVCKGESHWVDDDCEEIKAAECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHSPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGTFFWSVVIPHERRILTILQWLTLPDHERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIEDIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEERHLLYGRPAVLYRTRYDILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNEESCNSSEDESKWVEELMKMHTARVRDIEHLTSLDFFRKTSRSYPEILTLKTYLHTYESEI

Proteomics (Proteome ID):

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) (EC 3.1.4.39) (Autotaxin) (Extracellular lysophospholipase D) (LysoPLD)

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

N/A

Function [CC]:

Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931). Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931). Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor (PubMed:1733949, PubMed:11559573). {ECO:0000269|PubMed:11559573, ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:15769751, ECO:0000269|PubMed:1733949, ECO:0000269|PubMed:21240271, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000305|PubMed:15700135}.

Metal Binding:

METAL 172 172 Zinc 1; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 210 210 Zinc 1; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 312 312 Zinc 2; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000269|PubMed:26371182}.; METAL 316 316 Zinc 2; via tele nitrogen; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000269|PubMed:26371182}.; METAL 359 359 Zinc 1; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 360 360 Zinc 1; via tele nitrogen; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 475 475 Zinc 2; via tele nitrogen; catalytic. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000269|PubMed:26371182}.; METAL 740 740 Calcium. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 742 742 Calcium. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 744 744 Calcium. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000269|PubMed:26371182}.; METAL 746 746 Calcium; via carbonyl oxygen. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.; METAL 748 748 Calcium. {ECO:0000244|PDB:4ZG6, ECO:0000244|PDB:4ZG7, ECO:0000244|PDB:4ZG9, ECO:0000244|PDB:4ZGA, ECO:0000244|PDB:5KXA, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}.

Site:

SITE 853 853 Essential for catalytic activity. {ECO:0000250|UniProtKB:Q9R1E6}.

Tissue Specificity:

Detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:8579579, ECO:0000269|PubMed:8586446}.

Disease:

N/A

Mutagenesis:

MUTAGEN 170 170 S->E: Reduces lysophospholipase activity by about 70%.; MUTAGEN 210 210 T->A: Loss of lysophospholipase activity. {ECO:0000269|PubMed:21240271}.; MUTAGEN 211 211 F->Y: Reduces lysophospholipase activity by about 70%. {ECO:0000269|PubMed:21240271}.; MUTAGEN 218 218 A->V: Reduces lysophospholipase activity by about 50%. {ECO:0000269|PubMed:21240271}.; MUTAGEN 231 231 N->A: Strongly reduced lysophospholipase activity. {ECO:0000269|PubMed:21240271}.; MUTAGEN 307 307 Y->Q: Reduces lysophospholipase activity by about 70%. {ECO:0000269|PubMed:21240271}.

Name:

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) (EC 3.1.4.39) (Autotaxin) (Extracellular lysophospholipase D) (LysoPLD)

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.