Dkk-1

Gene Synonyms:

Protein Names:

Organism:

Mus musculus (Mouse)

Mass (kDa):

29298

Length (aa):

272

Sequence:

MMVVCAAAAVRFLAVFTMMALCSLPLLGASATLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKRKGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH

Proteomics (Proteome ID):

Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (mDkk-1)

Proteomics (Chromosome):

UP000000589

Mass Spectrometry:

N/A

Function [CC]:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed:18505822). {ECO:0000269|PubMed:18505822, ECO:0000269|PubMed:18524778, ECO:0000269|PubMed:26206087}.

Metal Binding:

N/A

Site:

N/A

Tissue Specificity:

N/A

Disease:

N/A

Mutagenesis:

MUTAGEN 210 210 H->E: 50% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.; MUTAGEN 217 217 K->E: 50% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.; MUTAGEN 242 242 R->E: 50% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.; MUTAGEN 267 267 H->E: 43% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.

Name:

Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (mDkk-1)

Class:

Subcategory:

Recombinant

Source:

Species:

Mouse

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.