DPPIV

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

88279

Length (aa):

766

Sequence:

MKTPWKVLLGLLGAAALVTIITVPVVLLNKGTDDATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

Proteomics (Proteome ID):

Dipeptidyl peptidase 4 (EC 3.4.14.5) (ADABP) (Adenosine deaminase complexing protein 2) (ADCP-2) (Dipeptidyl peptidase IV) (DPP IV) (T-cell activation antigen CD26) (TP103) (CD antigen CD26) [Cleaved into: Dipeptidyl peptidase 4 membrane form (Dipeptidyl peptidase IV membrane form); Dipeptidyl peptidase 4 soluble form (Dipeptidyl peptidase IV soluble form)]

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

N/A

Function [CC]:

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. {ECO:0000269|PubMed:10570924, ECO:0000269|PubMed:10593948, ECO:0000269|PubMed:10900005, ECO:0000269|PubMed:10951221, ECO:0000269|PubMed:11772392, ECO:0000269|PubMed:14691230, ECO:0000269|PubMed:16651416, ECO:0000269|PubMed:17287217, ECO:0000269|PubMed:17549790, ECO:0000269|PubMed:18708048}.

Metal Binding:

N/A

Site:

N/A

Tissue Specificity:

Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. {ECO:0000269|PubMed:1677636, ECO:0000269|PubMed:18708048}.

Disease:

N/A

Mutagenesis:

MUTAGEN 85 85 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 92 92 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 150 150 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 205 205 E->K: Inhibits dipeptidyl peptidase activity. {ECO:0000269|PubMed:10570924}.; MUTAGEN 206 206 E->L: Inhibits dipeptidyl peptidase activity. {ECO:0000269|PubMed:10570924}.; MUTAGEN 219 219 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 229 229 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 281 281 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 321 321 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 520 520 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 685 685 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 750 750 H->A: Inhibits weakly homodimerization and dipeptidyl peptidase activity. {ECO:0000269|PubMed:15448155, ECO:0000269|PubMed:17287217}.; MUTAGEN 750 750 H->E: Inhibits strongly homodimerization, dipeptidyl peptidase activity, interaction with CARD11 and T-cell costimulation activity. {ECO:0000269|PubMed:15448155, ECO:0000269|PubMed:17287217}.

Name:

Dipeptidyl peptidase 4 (EC 3.4.14.5) (ADABP) (Adenosine deaminase complexing protein 2) (ADCP-2) (Dipeptidyl peptidase IV) (DPP IV) (T-cell activation antigen CD26) (TP103) (CD antigen CD26) [Cleaved into: Dipeptidyl peptidase 4 membrane form (Dipeptidyl peptidase IV membrane form); Dipeptidyl peptidase 4 soluble form (Dipeptidyl peptidase IV soluble form)]

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.