Alpha Crystallin A

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

19909

Length (aa):

173

Sequence:

MDVTIQHPWFKRTLGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVQDDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFCGPKIQTGLDATHAERAIPVSREEKPTSAPSS

Proteomics (Proteome ID):

Alpha-crystallin A chain (Heat shock protein beta-4) (HspB4) [Cleaved into: Alpha-crystallin A(1-172); Alpha-crystallin A(1-168); Alpha-crystallin A(1-162)]

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

Mass=19950; Method=Electrospray; Range=1-173; Evidence={ECO:0000269|PubMed:8175657}; Mass=19863; Method=Electrospray; Range=1-172; Evidence={ECO:0000269|PubMed:8175657}; Mass=20029; Method=Electrospray; Range=1-173; Note=With 1 phosphate group.; Evidence={ECO:0000269|PubMed:8175657}; Mass=19951; Method=Electrospray; Range=1-173; Evidence={ECO:0000269|PubMed:9655350}; Mass=19864; Method=Electrospray; Range=1-172; Evidence={ECO:0000269|PubMed:9655350}; Mass=19947; Method=Electrospray; Range=1-173; Evidence={ECO:0000269|PubMed:10930324}; Mass=19851; Method=Electrospray; Range=1-172; Evidence={ECO:0000269|PubMed:10930324};

Function [CC]:

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. {ECO:0000269|PubMed:22120592}.

Metal Binding:

METAL 79 79 Zinc 1. {ECO:0000305}.; METAL 100 100 Zinc 2. {ECO:0000250}.; METAL 102 102 Zinc 2. {ECO:0000250}.; METAL 107 107 Zinc 1. {ECO:0000305}.; METAL 115 115 Zinc 1. {ECO:0000305}.

Site:

SITE 1 1 Susceptible to oxidation.; SITE 18 18 Susceptible to oxidation.; SITE 34 34 Susceptible to oxidation.; SITE 138 138 Susceptible to oxidation.

Tissue Specificity:

Expressed in eye lens. {ECO:0000269|PubMed:12356833}.

Disease:

Note=Alpha-crystallin A 1-172 is found at nearly twofold higher levels in diabetic lenses than in age-matched control lenses. {ECO:0000269|PubMed:12356833}.; Cataract 9, multiple types (CTRCT9) [MIM:604219]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. CTRCT9 includes nuclear, zonular central nuclear, anterior polar, cortical, embryonal, anterior subcapsular, fan-shaped, and total cataracts, among others. In some cases cataract is associated with microcornea without any other systemic anomaly or dysmorphism. Microcornea is defined by a corneal diameter inferior to 10 mm in both meridians in an otherwise normal eye. {ECO:0000269|PubMed:11123904, ECO:0000269|PubMed:14512969, ECO:0000269|PubMed:16453125, ECO:0000269|PubMed:18302245, ECO:0000269|PubMed:18407550, ECO:0000269|PubMed:23508780, ECO:0000269|PubMed:9467006}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Mutagenesis:

MUTAGEN 123 123 N->D: Impairs chaperone activity. {ECO:0000269|PubMed:18754677}.

Name:

Alpha-crystallin A chain (Heat shock protein beta-4) (HspB4) [Cleaved into: Alpha-crystallin A(1-172); Alpha-crystallin A(1-168); Alpha-crystallin A(1-162)]

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Solution

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

20mM

Buffer Solution:

Tris-HCl

pH:

7.5

NaCl:

50mM

EDTA:

1mM

Purity:

> 95%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

4°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.