PD-1

Gene Synonyms:

Protein Names:

Organism:

Mus musculus (Mouse)

Mass (kDa):

31842

Length (aa):

288

Sequence:

MWVRQVPWSFTWAVLQLSWQSGWLLEVPNGPWRSLTFYPAWLTVSEGANATFTCSLSNWSEDLMLNWNRLSPSNQTEKQAAFCNGLSQPVQDARFQIIQLPNRHDFHMNILDTRRNDSGIYLCGAISLHPKAKIEESPGAELVVTERILETSTRYPSPSPKPEGRFQGMVIGIMSALVGIPVLLLLAWALAVFCSTSMSEARGAGSKDDTLKEEPSAAPVPSVAYEELDFQGREKTPELPTACVHTEYATIVFTEGLGASAMGRRGSADGLQGPRPPRHEDGHCSWPL

Proteomics (Proteome ID):

Programmed cell death protein 1 (Protein PD-1) (mPD-1) (CD antigen CD279)

Proteomics (Chromosome):

UP000000589

Mass Spectrometry:

N/A

Function [CC]:

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11698646, PubMed:11209085, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:22641383, PubMed:18287011, PubMed:18641123). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity). {ECO:0000250|UniProtKB:Q15116, ECO:0000269|PubMed:10485649, ECO:0000269|PubMed:11015443, ECO:0000269|PubMed:11209085, ECO:0000269|PubMed:11224527, ECO:0000269|PubMed:11698646, ECO:0000269|PubMed:18287011, ECO:0000269|PubMed:18641123, ECO:0000269|PubMed:21300912, ECO:0000269|PubMed:22641383}.

Metal Binding:

N/A

Site:

N/A

Tissue Specificity:

Thymus-specific. {ECO:0000269|PubMed:1396582}.

Disease:

N/A

Mutagenesis:

MUTAGEN 225 225 Y->F: Reduced tyrosine phosphorylation. Decreased ability to mediate recruitment of PTPN11/SHP-2. {ECO:0000269|PubMed:11698646, ECO:0000269|PubMed:22641383}.; MUTAGEN 248 248 Y->F: Reduced tyrosine phosphorylation. Abolished ability to mediate recruitment of PTPN11/SHP-2. {ECO:0000269|PubMed:11698646, ECO:0000269|PubMed:22641383}.

Name:

Programmed cell death protein 1 (Protein PD-1) (mPD-1) (CD antigen CD279)

Class:

Subcategory:

Recombinant

Source:

Species:

Mouse

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.