Hemagglutinin

Gene Synonyms:

Protein Names:

Organism:

Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1))

Mass (kDa):

49053

Length (aa):

449

Sequence:

MNPNKKIITIGSICMVTGMVSLMLQIGNLISIWVSHSIHTGNQHKAEPISNTNFLTEKAVASVKLAGNSSLCPINGWAVYSKDNSIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWSASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQASHKIFKMEKGKVVKSVELDAPNYHYEECSCYPDAGEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSSNGAYGVKGFSFKYGNGVWIGRTKSTNSRSGFEMIWDPNGWTETDSSFSVKQDIVAITDWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKESTIWTSGSSISFCGVTSDTVGWSWPDGAELPFTIDK

Proteomics (Proteome ID):

Neuraminidase (EC 3.2.1.18)

Proteomics (Chromosome):

UP000126071

Mass Spectrometry:

N/A

Function [CC]:

Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication. {ECO:0000256|HAMAP-Rule:MF_04071, ECO:0000256|SAAS:SAAS00844152}.

Metal Binding:

METAL 274 274 Calcium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_04071}.; METAL 278 278 Calcium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_04071}.; METAL 304 304 Calcium. {ECO:0000256|HAMAP-Rule:MF_04071}.

Site:

N/A

Tissue Specificity:

N/A

Disease:

N/A

Mutagenesis:

N/A

Name:

Neuraminidase (EC 3.2.1.18)

Class:

Subcategory:

Recombinant

Source:

Species:

Influenza

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.