LTA4H
Recombinant ID:
3926
Request Datasheet
Gene of Interest
Gene Synonyms:
Protein Names:
Accession Data
Organism:
Homo sapiens (Human)
Mass (kDa):
69285
Length (aa):
611
Sequence:
MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNLRSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD
Proteomics (Proteome ID):
Leukotriene A-4 hydrolase (LTA-4 hydrolase) (EC 3.3.2.6) (Leukotriene A(4) hydrolase)
Proteomics (Chromosome):
UP000005640
Mass Spectrometry:
N/A
Function [CC]:
Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:1897988, ECO:0000269|PubMed:1975494, ECO:0000269|PubMed:2244921}.
Metal Binding:
METAL 296 296 Zinc; catalytic. {ECO:0000244|PDB:1GW6, ECO:0000244|PDB:1H19, ECO:0000244|PDB:1HS6, ECO:0000244|PDB:1SQM, ECO:0000244|PDB:2R59, ECO:0000244|PDB:2VJ8, ECO:0000244|PDB:3B7R, ECO:0000244|PDB:3B7S, ECO:0000244|PDB:3B7T, ECO:0000244|PDB:3B7U, ECO:0000244|PDB:3CHO, ECO:0000244|PDB:3CHP, ECO:0000244|PDB:3CHQ, ECO:0000244|PDB:3CHR, ECO:0000244|PDB:3CHS, ECO:0000244|PDB:3FH5, ECO:0000244|PDB:3FH7, ECO:0000244|PDB:3FH8, ECO:0000244|PDB:3FHE, ECO:0000244|PDB:3FTS, ECO:0000244|PDB:3FTU, ECO:0000244|PDB:3FTV, ECO:0000244|PDB:3FTW, ECO:0000244|PDB:3FTX, ECO:0000244|PDB:3FTY, ECO:0000244|PDB:3FTZ, ECO:0000244|PDB:3FU0, ECO:0000244|PDB:3FU3, ECO:0000244|PDB:3FU5, ECO:0000244|PDB:3FU6, ECO:0000244|PDB:3FUD, ECO:0000244|PDB:3FUE, ECO:0000244|PDB:3FUF, ECO:0000244|PDB:3FUH, ECO:0000244|PDB:3FUI, ECO:0000244|PDB:3FUJ, ECO:0000244|PDB:3FUK, ECO:0000244|PDB:3FUL, ECO:0000244|PDB:3FUM, ECO:0000244|PDB:3FUN, ECO:0000244|PDB:3U9W, ECO:0000244|PDB:4DPR, ECO:0000244|PDB:4L2L, ECO:0000244|PDB:4MKT, ECO:0000244|PDB:4MS6, ECO:0000244|PDB:5AEN, ECO:0000269|PubMed:11175901, ECO:0000269|PubMed:11675384, ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:19618939}.; METAL 300 300 Zinc; catalytic. {ECO:0000244|PDB:1GW6, ECO:0000244|PDB:1H19, ECO:0000244|PDB:1HS6, ECO:0000244|PDB:1SQM, ECO:0000244|PDB:2R59, ECO:0000244|PDB:2VJ8, ECO:0000244|PDB:3B7R, ECO:0000244|PDB:3B7S, ECO:0000244|PDB:3B7T, ECO:0000244|PDB:3B7U, ECO:0000244|PDB:3CHO, ECO:0000244|PDB:3CHP, ECO:0000244|PDB:3CHQ, ECO:0000244|PDB:3CHR, ECO:0000244|PDB:3CHS, ECO:0000244|PDB:3FH5, ECO:0000244|PDB:3FH7, ECO:0000244|PDB:3FH8, ECO:0000244|PDB:3FHE, ECO:0000244|PDB:3FTS, ECO:0000244|PDB:3FTU, ECO:0000244|PDB:3FTV, ECO:0000244|PDB:3FTW, ECO:0000244|PDB:3FTX, ECO:0000244|PDB:3FTY, ECO:0000244|PDB:3FTZ, ECO:0000244|PDB:3FU0, ECO:0000244|PDB:3FU3, ECO:0000244|PDB:3FU5, ECO:0000244|PDB:3FU6, ECO:0000244|PDB:3FUD, ECO:0000244|PDB:3FUE, ECO:0000244|PDB:3FUF, ECO:0000244|PDB:3FUH, ECO:0000244|PDB:3FUI, ECO:0000244|PDB:3FUJ, ECO:0000244|PDB:3FUK, ECO:0000244|PDB:3FUL, ECO:0000244|PDB:3FUM, ECO:0000244|PDB:3FUN, ECO:0000244|PDB:3U9W, ECO:0000244|PDB:4DPR, ECO:0000244|PDB:4L2L, ECO:0000244|PDB:4MKT, ECO:0000244|PDB:4MS6, ECO:0000244|PDB:5AEN, ECO:0000269|PubMed:11175901, ECO:0000269|PubMed:11675384, ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:19618939}.; METAL 319 319 Zinc; catalytic. {ECO:0000244|PDB:1GW6, ECO:0000244|PDB:1H19, ECO:0000244|PDB:1HS6, ECO:0000244|PDB:1SQM, ECO:0000244|PDB:2R59, ECO:0000244|PDB:2VJ8, ECO:0000244|PDB:3B7R, ECO:0000244|PDB:3B7S, ECO:0000244|PDB:3B7T, ECO:0000244|PDB:3B7U, ECO:0000244|PDB:3CHO, ECO:0000244|PDB:3CHP, ECO:0000244|PDB:3CHQ, ECO:0000244|PDB:3CHR, ECO:0000244|PDB:3CHS, ECO:0000244|PDB:3FH5, ECO:0000244|PDB:3FH7, ECO:0000244|PDB:3FH8, ECO:0000244|PDB:3FHE, ECO:0000244|PDB:3FTS, ECO:0000244|PDB:3FTU, ECO:0000244|PDB:3FTV, ECO:0000244|PDB:3FTW, ECO:0000244|PDB:3FTX, ECO:0000244|PDB:3FTY, ECO:0000244|PDB:3FTZ, ECO:0000244|PDB:3FU0, ECO:0000244|PDB:3FU3, ECO:0000244|PDB:3FU5, ECO:0000244|PDB:3FU6, ECO:0000244|PDB:3FUD, ECO:0000244|PDB:3FUE, ECO:0000244|PDB:3FUF, ECO:0000244|PDB:3FUH, ECO:0000244|PDB:3FUI, ECO:0000244|PDB:3FUJ, ECO:0000244|PDB:3FUK, ECO:0000244|PDB:3FUL, ECO:0000244|PDB:3FUM, ECO:0000244|PDB:3FUN, ECO:0000244|PDB:3U9W, ECO:0000244|PDB:4DPR, ECO:0000244|PDB:4L2L, ECO:0000244|PDB:4MKT, ECO:0000244|PDB:4MS6, ECO:0000244|PDB:5AEN, ECO:0000269|PubMed:11175901, ECO:0000269|PubMed:11675384, ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:19618939}.
Site:
SITE 376 376 Essential for epoxide hydrolase activity, but not for aminopeptidase activity.; SITE 379 379 Covalently modified during suicide inhibition by leukotrienes.
Tissue Specificity:
Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
Disease:
N/A
Mutagenesis:
MUTAGEN 135 135 Q->A,L: Srongly increased epoxide hydrolase activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 135 135 Q->A: Strongly reduced aminopeptidase activity. Strongly decreased affinity for leukotriene. Abolishes epoxide hydrolase activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 137 137 Q->A: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 137 137 Q->L: Aminopeptidase activity strongly impaired, but keeps LTA4 activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 137 137 Q->N: Aminopeptidase activity almost absent, but keeps LTA4 activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 140 140 H->Q: Aminopeptidase activity almost absent, but keeps LTA4 activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 269 269 G->A: No loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 270 270 G->A: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 271 271 M->L: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 272 272 E->A,D: Complete loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 272 272 E->Q: Loss of LTA4 activity, and aminopeptidase activity strongly impaired. {ECO:0000269|PubMed:11675384}.; MUTAGEN 273 273 N->A: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 296 296 H->Y: Complete loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 297 297 E->A: Loss of both activities. {ECO:0000269|PubMed:1357660, ECO:0000269|PubMed:1516710, ECO:0000269|PubMed:18804029}.; MUTAGEN 297 297 E->K: Loss of both activities. {ECO:0000269|PubMed:1357660, ECO:0000269|PubMed:1516710, ECO:0000269|PubMed:18804029}.; MUTAGEN 297 297 E->Q: Loss of aminopeptidase activity, but keeps LTA4 activity. {ECO:0000269|PubMed:1357660, ECO:0000269|PubMed:1516710, ECO:0000269|PubMed:18804029}.; MUTAGEN 300 300 H->L: Complete loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 319 319 E->A: Complete loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 372 372 D->N: No loss of activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 374 374 D->N: No loss of activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 376 376 D->A: Strongly reduced hydrolysis of peptides starting with Arg. Small effect on hydrolysis of peptides starting with Ala. Strongly reduced epoxide hydrolase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:18804029}.; MUTAGEN 376 376 D->E: Strongly reduced aminopeptidase activity. Abolishes epoxide hydrolase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:18804029}.; MUTAGEN 376 376 D->N: Abolishes aminopeptidase and epoxide hydrolase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:18804029}.; MUTAGEN 385 385 E->Q: Reduced aminopeptidase activity. Minor effect on epoxide hydrolase activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 564 564 R->A,K,M: Abolishes epoxide hydrolase activity. Reduced aminopeptidase activity. {ECO:0000269|PubMed:15078870}.; MUTAGEN 566 566 K->A,M: Strongly reduced affinity for peptide substrates. Reduced epoxide hydrolase and aminopeptidase activity. {ECO:0000269|PubMed:15078870}.; MUTAGEN 566 566 K->R: No effect on epoxide hydrolase and aminopeptidase activity. {ECO:0000269|PubMed:15078870}.
Reagent Data
Name:
Leukotriene A-4 hydrolase (LTA-4 hydrolase) (EC 3.3.2.6) (Leukotriene A(4) hydrolase)
Class:
Subcategory:
Recombinant
Molecular Weight:
Source:
Species:
Human
Amino Acid Sequence:
Tag:
Format:
Lyophilized
Formulation:
Sterile-filtered colorless solution
Formulation Concentration:
1mg/ml
Buffer Volume:
Standard
Buffer Solution:
PBS
pH:
7.4-7.5
Stabilizers
NaCl:
Null
Metal Chelating Agents
EDTA:
Null
Purity:
> 98%
Determined:
SDS-PAGE
Stained:
Inquire
Validated:
RP-HPLC
Sample Handling
Storage:
-20°C
Stability:
This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:
Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
