Carboxypeptidase M

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

50514

Length (aa):

443

Sequence:

MDFPCLWLGLLLPLVAALDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHSAATKPSLFLFLVSLLHIFFK

Proteomics (Proteome ID):

Carboxypeptidase M (CPM) (EC 3.4.17.12)

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

N/A

Function [CC]:

Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. {ECO:0000269|PubMed:12457462}.

Metal Binding:

METAL 83 83 Zinc; catalytic. {ECO:0000244|PDB:1UWY, ECO:0000269|PubMed:15066430}.; METAL 86 86 Zinc; catalytic. {ECO:0000244|PDB:1UWY, ECO:0000269|PubMed:15066430}.; METAL 190 190 Zinc; catalytic. {ECO:0000244|PDB:1UWY, ECO:0000269|PubMed:15066430}.

Site:

SITE 277 277 Probable structural role. {ECO:0000305|PubMed:12457462}.

Tissue Specificity:

N/A

Disease:

N/A

Mutagenesis:

MUTAGEN 277 277 E->A: 5-fold decrease in substrate affinity. 22-fold decrease in specific affinity. 104-fold decrease in catalytic efficiency. Greatly reduced heat stability. {ECO:0000269|PubMed:12457462}.; MUTAGEN 277 277 E->Q: 2-fold decrease in substrate affinity. Small increase in specific affinity. Reduced heat stability by 50%. {ECO:0000269|PubMed:12457462}.; MUTAGEN 281 281 E->Q: Abolishes enzyme activity. {ECO:0000269|PubMed:12457462}.; MUTAGEN 423 423 S->A,T: Expressed on cell membrane. Released from membrane by PI-PLC. {ECO:0000269|PubMed:12457462}.; MUTAGEN 423 423 S->P: Little expression on cell membrane. Perinuclear localization. Not released from membrane by PI-PLC. {ECO:0000269|PubMed:12457462}.

Name:

Carboxypeptidase M (CPM) (EC 3.4.17.12)

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.