PD-L1

Gene Synonyms:

Protein Names:

Organism:

Mus musculus (Mouse)

Mass (kDa):

32780

Length (aa):

290

Sequence:

MRIFAGIIFTACCHLLRAFTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAELIIPELPATHPPQNRTHWVLLGSILLFLIVVSTVLLFLRKQVRMLDVEKCGVEDTSSKNRNDTQFEET

Proteomics (Proteome ID):

Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)

Proteomics (Chromosome):

UP000000589

Mass Spectrometry:

N/A

Function [CC]:

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11238124). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11238124). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:11015443, PubMed:12719480). {ECO:0000269|PubMed:11015443, ECO:0000269|PubMed:11238124, ECO:0000269|PubMed:12719480}.; The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:12218188, PubMed:27281199). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:12218188). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:12218188). {ECO:0000269|PubMed:12218188, ECO:0000269|PubMed:27281199}.

Metal Binding:

N/A

Site:

N/A

Tissue Specificity:

Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin. {ECO:0000269|PubMed:11015443, ECO:0000269|PubMed:11224527, ECO:0000269|PubMed:11238124}.

Disease:

N/A

Mutagenesis:

MUTAGEN 27 27 L->A: PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 31 31 E->S: Significantly reduces the binding to PDCD1. {ECO:0000269|PubMed:12719480}.; MUTAGEN 34 34 S->Y: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 37 37 T->Y: Significantly reduces the binding to PDCD1. {ECO:0000269|PubMed:12719480}.; MUTAGEN 49 49 D->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 56 56 Y->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 58 58 E->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 62 62 E->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 67 67 F->A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. {ECO:0000269|PubMed:12719480}.; MUTAGEN 69 69 A->F: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 72 72 E->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 75 75 K->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 89 89 K->S: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 98 98 A->F: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 100 100 Q->S: No effect on PDCD1 binding.; MUTAGEN 113 113 C->Y: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 115 115 I->A: Abolishes the binding to PDCD1. {ECO:0000269|PubMed:12719480}.; MUTAGEN 117 117 S->Y: No effect on PDCD1 binding. {ECO:0000269|PubMed:12719480}.; MUTAGEN 124 124 K->A: Abolishes the binding to PDCD1. {ECO:0000269|PubMed:12719480}.; MUTAGEN 126 126 I->A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. {ECO:0000269|PubMed:12719480}.; MUTAGEN 129 129 K->S: Abolishes the binding to PDCD1. {ECO:0000269|PubMed:12719480}.

Name:

Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274)

Class:

Subcategory:

Recombinant

Source:

Species:

Mouse

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.