Furin

Gene Synonyms:

Protein Names:

Organism:

Homo sapiens (Human)

Mass (kDa):

86678

Length (aa):

794

Sequence:

MELRPWLLWVVAATGTLVLLAADAQGQKVFTNTWAVRIPGGPAVANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAKRRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRSGFSFRGVKVYTMDRGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL

Proteomics (Proteome ID):

Furin (EC 3.4.21.75) (Dibasic-processing enzyme) (Paired basic amino acid residue-cleaving enzyme) (PACE)

Proteomics (Chromosome):

UP000005640

Mass Spectrometry:

N/A

Function [CC]:

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771, PubMed:2251280, PubMed:24666235, PubMed:25974265, PubMed:7592877, PubMed:7690548, PubMed:9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed:7737999). {ECO:0000269|PubMed:11799113, ECO:0000269|PubMed:1629222, ECO:0000269|PubMed:1713771, ECO:0000269|PubMed:2251280, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265, ECO:0000269|PubMed:7592877, ECO:0000269|PubMed:7690548, ECO:0000269|PubMed:7737999, ECO:0000269|PubMed:9130696}.; (Microbial infection) Probably cleaves and activates anthrax and diphtheria toxins.; (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin. {ECO:0000269|PubMed:25974265}.; (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP. {ECO:0000269|PubMed:27582320}.

Metal Binding:

METAL 115 115 Calcium 1. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 162 162 Calcium 1. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 174 174 Calcium 2. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 179 179 Calcium 2. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 181 181 Calcium 2; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 205 205 Calcium 1; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 208 208 Calcium 1. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 210 210 Calcium 1; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 212 212 Calcium 1; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 258 258 Calcium 3. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 301 301 Calcium 3. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 331 331 Calcium 3. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.

Site:

SITE 75 76 Cleavage, second; by autolysis. {ECO:0000269|PubMed:9130696}.; SITE 107 108 Cleavage, first; by autolysis. {ECO:0000269|PubMed:1629222, ECO:0000269|PubMed:9130696}.

Tissue Specificity:

Seems to be expressed ubiquitously. {ECO:0000269|PubMed:1713771}.

Disease:

N/A

Mutagenesis:

MUTAGEN 72 72 V->R: Loss of catalytic activity and propeptide second cleavage and removal. Abnormal accumulation in the early secretory pathway. {ECO:0000269|PubMed:11799113}.; MUTAGEN 75 75 R->A: Loss of catalytic activity and, propeptide second cleavage and removal. Normal trafficking to the Golgi. {ECO:0000269|PubMed:11799113}.; MUTAGEN 153 153 D->N: Loss of catalytic activity and propeptide first cleavage. Abnormal accumulation in the early secretory pathway. {ECO:0000269|PubMed:11799113, ECO:0000269|PubMed:9130696}.; MUTAGEN 773 775 SDS->DDD: Phosphomimetic mutant. Localization in early endosome is increased. {ECO:0000269|PubMed:8846780}.; MUTAGEN 773 773 S->A: Slight reduction in phosphorylation. Loss of phosphorylation and abnormal accumulation in the early secretory pathway; when associated with A-775. {ECO:0000269|PubMed:8846780}.; MUTAGEN 775 775 S->A: Slight reduction in phosphorylation. Loss of phosphorylation and abnormal accumulation in the early secretory pathway; when associated with A-773. {ECO:0000269|PubMed:8846780}.

Name:

Furin (EC 3.4.21.75) (Dibasic-processing enzyme) (Paired basic amino acid residue-cleaving enzyme) (PACE)

Class:

Subcategory:

Recombinant

Source:

Species:

Human

Amino Acid Sequence:

Tag:

Format:

Lyophilized

Formulation:

Sterile-filtered colorless solution

Formulation Concentration:

1mg/ml

Buffer Volume:

Standard

Buffer Solution:

PBS

pH:

7.4-7.5

NaCl:

Null

EDTA:

Null

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Inquire

Validated:

RP-HPLC

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.