DNase I is an essential endonuclease enzyme used in laboratories and clinical settings for RNA purification. It efficiently hydrolyzes the phosphodiester bonds in DNA, including single-stranded and double-stranded DNA, chromatin, and RNA:DNA hybrids, guaranteeing the isolation of RNA samples that are entirely free from DNA contamination. High-quality GMP Grade DNase I is available to meet the rigorous standards required for research, pre-clinical, and clinical applications.

Advantages:

• Manufactured in accordance with GMP rules and regulations
• 100% animal origin free manufacturing process
• Endotoxin and antibiotic free
• Complete QA documentation available

• Removal of DNA templates from In-vitro transcription reactions
• Preparation of DNA-free templates for RT-PCR
• Manufacture of mRNA vaccines
• Manufacture of mRNA therapeutics
• RNA-based diagnostic assays
• Preparation of RNA probes or guide RNA

• mRNA preparation for micro injection and in-vitro translation
• RNA structure, processing and catalysis studies
• Host Cell DNA removal in Biosimilars manufacturing
• Viscosity reduction in cell lysis and downstream process of protein purifications
• Viral vaccine purifications

EC Number:

3.1.21.1

Source:

Recombinant E.Coli

 Molecular Weight:

39 kd (untagged)

79 kDa (tagged)

Optimum pH:

7.0 - 8.0

Activators:

Bivalent cations

Inhibitors:

EDTA, EGTA, SDS

Appearance:

Colorless to yellowish solution

Volume Activity:

5-10 kU/mL

Unit Definition:

One unit is defined as the amount of enzyme which will completely degrade 1 μg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer

Specific Activity:

NLT 10000Units per mg of protein

Ribonucleases:

Not detectable

Proteases:

Not detectable

Stability:

-15 to -25°C within specification range for 24 months from date of manufacture.

Storage:

Enzyme supplied in storage buffer of composition:
10 mM Tris-HCl, 2 mM CaCl2, 50% Glycerol, pH 7.6 @ 25°C

Buffer:

10X reaction Buffer will be provided.

Unit Definition:

One unit is defined as the amount of enzyme which will completely degrade 1 μg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer

Notes on use:

5 mM EDTA should be added to protect RNA degradation during enzyme inactivation

Method of Analysis will be provided on Bulk Enzyme orders for customer’s in-house quality testing