IL-10
Recombinant ID:
3603
Gene of Interest
Gene Synonyms:
Il10;Il-10
Protein Names:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Accession Data
Organism:
Mus musculus (Mouse)
Mass (kDa):
20641
Length (aa):
178
Proteomics (Proteome ID):
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Proteomics (Chromosome):
UP000000589
Sequence:
MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS
Analysis Summary:
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584). {ECO:0000250|UniProtKB:P22301, ECO:0000269|PubMed:28473584}.,Chain (1); Disulfide bond (2); Glycosylation (2); Helix (11); Signal peptide (1); Turn (1)
Reagent Data
Name:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Subcategory:
Recombinant
Amino Acid Sequence:
Inquire
Species
Mouse
Tag:
Format:
Lyophilized
pH:
7.4-7.5
Formulation:
Sterile-filtered colorless solution
Buffer Volume
Standard
Buffer Solution:
PBS
Metal Chelating Agents
Purity:
> 98%
Determined:
SDS-PAGE
Stained:
Assay (Variable)
Validated:
RP-HPLC
Sample Handling
Storage:
-20°C
Stability:
This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:
Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
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