Glypican 3
Recombinant ID:
3368
Gene of Interest
Gene Synonyms:
Gpc3
Protein Names:
Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
Accession Data
Organism:
Mus musculus (Mouse)
Mass (kDa):
65332
Length (aa):
579
Metal Binding:
Pending Verification
Proteomics (Proteome ID):
Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
Proteomics (Chromosome):
UP000000589
Disease:
N/A
Mutagenesis:
N/A
Sequence:
MAGTVRTACLLVAMLLGLGCLGQAQPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQMMNPGLPESVLDINECLRGARRDLKVFGSFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNGGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKILKVAHVEHEETLSSRRRELIQKLKSFINFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSVPKGKVLDKSLDEEGLESGDCGDDEDECIGSSGDGMVKVKNQLRFLAELAYDLDVDDAPGNKQHGNQKDNEITTSHSVGNMPSPLKILISVAIYVACFFFLVH
Function [CC]:
Cell surface proteoglycan that bears heparan sulfate (By similarity). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (PubMed:18477453, PubMed:23665349). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (PubMed:18477453). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (By similarity). Positively regulates the non-canonical Wnt signaling pathway (PubMed:15537637). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (PubMed:10964473). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (PubMed:11180950). Required for coronary vascular development (PubMed:19733558). Plays a role in regulating cell movements during gastrulation (By similarity). {ECO:0000250|UniProtKB:P51654, ECO:0000250|UniProtKB:Q6V9Y8, ECO:0000269|PubMed:10964473, ECO:0000269|PubMed:11180950, ECO:0000269|PubMed:15537637, ECO:0000269|PubMed:18477453, ECO:0000269|PubMed:19733558, ECO:0000269|PubMed:23665349}.
Analysis Summary:
Chain (2); Disulfide bond (7); Glycosylation (5); Lipidation (1); Modified residue (2); Propeptide (1); Signal peptide (1); In the developing limb, absent from the apical epidermal ridge at 11 dpc but highly expressed in the underlying mesenchyme (PubMed:10964473). Expression in the mesenchyme at this stage is asymmetric with highest levels in the regions of the distal mesenchyme within the progress zone and within the proximal anterior and posterior limb bud (PubMed:10964473). At later developmental stages including 12.5 and 13.5 dpc, expression is restricted to the interdigital webs and the regions of chondrocytic differentiation of the developing bones (PubMed:10964473). In the embryonic kidney, expressed in both the ureteric bud and mesenchymal cells as early as 13.5 dpc (PubMed:11180950). Expression at 16.5 dpc is similar to that at 13.5 dpc but decreases by 18.5 dpc (PubMed:11180950). {ECO:0000269|PubMed:10964473, ECO:0000269|PubMed:11180950}.
Reagent Data
Name:
Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
Subcategory:
Recombinant
Source:
HEK293
Species:
Format:
Lyophilized
pH:
7.4-7.5
Formulation:
Sterile-filtered colorless solution
Formulation Concentration:
1mg/ml
Buffer Volume:
Standard
Buffer Solution:
PBS
Metal Chelating Agents
Determined:
SDS-PAGE
Purity:
> 98%
Validated:
RP-HPLC
Sample Handling
Storage:
-20°C
Stability:
This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:
Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
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