Dkk-1
Recombinant ID:
3292
Gene of Interest
Gene Synonyms:
Dkk1
Protein Names:
Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (mDkk-1)
Accession Data
Organism:
Mus musculus (Mouse)
Mass (kDa):
29298
Length (aa):
272
Metal Binding:
Pending Verification
Proteomics (Proteome ID):
Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (mDkk-1)
Proteomics (Chromosome):
UP000000589
Disease:
N/A
Mutagenesis:
MUTAGEN 210 210 H->E: 50% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.; MUTAGEN 217 217 K->E: 50% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.; MUTAGEN 242 242 R->E: 50% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.; MUTAGEN 267 267 H->E: 43% reduced binding to LRP6. {ECO:0000269|PubMed:18524778}.
Sequence:
MMVVCAAAAVRFLAVFTMMALCSLPLLGASATLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKRKGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH
Function [CC]:
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed:18505822). {ECO:0000269|PubMed:18505822, ECO:0000269|PubMed:18524778, ECO:0000269|PubMed:26206087}.
Analysis Summary:
Chain (1); Disulfide bond (10); Glycosylation (2); Mutagenesis (4); Region (2); Sequence conflict (2); Signal peptide (1); Expressed in the developing brain, cochlea and limb buds. {ECO:0000269|PubMed:26206087, ECO:0000269|PubMed:27550540}.
Reagent Data
Name:
Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (mDkk-1)
Subcategory:
Recombinant
Source:
HEK293
Species:
Format:
Lyophilized
pH:
7.4-7.5
Formulation:
Sterile-filtered colorless solution
Formulation Concentration:
1mg/ml
Buffer Volume:
Standard
Buffer Solution:
PBS
Metal Chelating Agents
Determined:
SDS-PAGE
Purity:
> 98%
Validated:
RP-HPLC
Sample Handling
Storage:
-20°C
Stability:
This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:
Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
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