PECAM1

Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)

Homo sapiens (Human)

82522

738

Pending Verification

Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)

N/A

MUTAGEN 52 52 N->Q: Probable loss of N-glycosylation. No effect on homophilic cell adhesion; when associated with Q-84 and Q-151. {ECO:0000269|PubMed:27958302}.; MUTAGEN 74 74 L->E: Reduced homophilic cell adhesion; when associated with E-112; E-188 and E-190. {ECO:0000269|PubMed:27958302}.; MUTAGEN 84 84 N->Q: Probable loss of N-glycosylation. No effect on homophilic cell adhesion; when associated with Q-52 and Q-151. {ECO:0000269|PubMed:27958302}.; MUTAGEN 89 89 K->A: Lacks homophilic binding ability and is distributed over the entire plasma membrane. {ECO:0000269|PubMed:19342684}.; MUTAGEN 112 112 I->E: Reduced homophilic cell adhesion; when associated with E-74; E-188 and E-190. {ECO:0000269|PubMed:27958302}.; MUTAGEN 151 151 N->Q: Probable loss of N-glycosylation. No effect on homophilic cell adhesion; when associated with Q-52 and Q-84. {ECO:0000269|PubMed:27958302}.; MUTAGEN 188 188 F->E: Reduced homophilic cell adhesion; when associated with E-74; E-112 and E-190. {ECO:0000269|PubMed:27958302}.; MUTAGEN 190 190 I->E: Reduced homophilic cell adhesion; when associated with E-74; E-112 and E-188. {ECO:0000269|PubMed:27958302}.; MUTAGEN 622 622 C->A: 6-fold decrease in association with membrane microdomains. {ECO:0000269|PubMed:17139370}.; MUTAGEN 690 690 Y->F: No effect on Tyr-713 phosphorylation. Inhibits targeted recycling of PECAM1 from the lateral border recycling compartment (LBRC) around transmigrating monocytes. Decreases phosphorylation. No effect on interaction with PTPN11. Loss of phosphorylation and loss of binding to PTPN11; when associated with F-713. {ECO:0000269|PubMed:18388311, ECO:0000269|PubMed:19342684, ECO:0000269|PubMed:21464369}.; MUTAGEN 713 713 Y->F: Loss of Tyr-690 phosphorylation. Does not inhibit targeted recycling of PECAM1 from the lateral border recycling compartment (LBRC) around transmigrating monocytes. Decreases phosphorylation. Loss of interaction with PTPN11. Loss of phosphorylation and loss of binding to PTPN11; when associated with F-690. {ECO:0000269|PubMed:18388311, ECO:0000269|PubMed:19342684, ECO:0000269|PubMed:21464369}.

MQPRWAQGATMWLGVLLTLLLCSSLEGQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). {ECO:0000250|UniProtKB:Q08481, ECO:0000269|PubMed:12110892, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18672896, ECO:0000269|PubMed:19342684, ECO:0000269|PubMed:27958302}.; Isoform Delta15: Does not protect against apoptosis. {ECO:0000269|PubMed:18388311}.

Alternative sequence (6); Beta strand (17); Chain (1); Disulfide bond (6); Domain (6); Glycosylation (9); Helix (2); Lipidation (1); Modified residue (4); Motif (2); Mutagenesis (11); Natural variant (5); Region (2); Sequence conflict (6); Signal peptide (1); Topological domain (2); Transmembrane (1); Turn (1); Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311). {ECO:0000269|PubMed:12433657, ECO:0000269|PubMed:17580308, ECO:0000269|PubMed:18388311, ECO:0000269|PubMed:19342684, ECO:0000269|PubMed:21464369}.

Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)

Recombinant

HEK293

Lyophilized

7.4-7.5

Sterile-filtered colorless solution

1mg/ml

Standard

PBS

SDS-PAGE

> 98%

RP-HPLC

-20°C

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.