Hemagglutinin
Influenza Recombinant NA - His Tagged
Reference ID:KB-3898
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:NA
Protein Names:Neuraminidase (EC 3.2.1.18)
Accession Data
Organism:Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1))
Mass (kDa):490.53
Length (aa):449
Sequence:MNPNKKIITIGSICMVTGMVSLMLQIGNLISIWVSHSIHTGNQHKAEPISNTNFLTEKAVASVKLAGNSSLCPINGWAVYSKDNSIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWSASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQASHKIFKMEKGKVVKSVELDAPNYHYEECSCYPDAGEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSSNGAYGVKGFSFKYGNGVWIGRTKSTNSRSGFEMIWDPNGWTETDSSFSVKQDIVAITDWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKESTIWTSGSSISFCGVTSDTVGWSWPDGAELPFTIDK
Proteomics (Proteome ID):UP000126071
Proteomics (Chromosome): Genome
Active Site:ACT_SITE 131 131 Proton donor/acceptor. {ECO:0000256|HAMAP-Rule:MF_04071}.; ACT_SITE 382 382 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_04071}.
Activity Regulation: Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare. {ECO:0000256|HAMAP-Rule:MF_04071, ECO:0000256|SAAS:SAAS01070481}.
Binding Site:BINDING 98 98 Substrate. {ECO:0000256|HAMAP-Rule:MF_04071}.; BINDING 132 132 Substrate. {ECO:0000256|HAMAP-Rule:MF_04071}.; BINDING 273 273 Substrate. {ECO:0000256|HAMAP-Rule:MF_04071}.; BINDING 348 348 Substrate. {ECO:0000256|HAMAP-Rule:MF_04071}.
Catalytic Activity: Reaction=Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.; EC=3.2.1.18; Evidence={ECO:0000256|HAMAP-Rule:MF_04071, ECO:0000256|RuleBase:RU361252, ECO:0000256|SAAS:SAAS01116071};
Cofactor:Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000256|HAMAP-Rule:MF_04071, ECO:0000256|RuleBase:RU361252, ECO:0000256|SAAS:SAAS00612833};
Function [CC]:Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication. {ECO:0000256|HAMAP-Rule:MF_04071, ECO:0000256|SAAS:SAAS00844152}.
Metal Binding:METAL 274 274 Calcium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_04071}.; METAL 278 278 Calcium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_04071}.; METAL 304 304 Calcium. {ECO:0000256|HAMAP-Rule:MF_04071}.
Chemical Profile | ChEBI:Ca(2+) [CHEBI:29108]
ChEBI (Cofactor):Ca(2+) [CHEBI:29108]
Reagent Data
Name:Hemagglutinin
Class:Infectious
Subcategory:Antigen
Region:His 36 - Lys 449
Molecular Weight:46.1
Source:HEK293
Species:Influenza
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.