Modifying DNA during synthesis involves a bit of creativity along with untraditional chemistry throughout the synthesis workflow. Different classes of modifiers have grown over time, but the most tried and true begins with 3’ or 5’ amino modifiers and the use of spacers and “branching” modifications. This paved the way for introducing more elaborate modifiers into sequences, such as compounded functional groups and a wide array of labels.



See the full list of in-house DNA Modifiers used in our synthesis below: 



DNA Modifiers
A: Chemical Phosphorylation and Supports
5'- Phosphorylation; Bis-cyanoethyl-N,N-diisopropyl CEP
3'-Phosphate-ON Icaa CPG
5-Phosphorylation (generates DMTr color)-(5-Phosphate-ON Reagent)
B: Amino Modifiers and Supports
3'-Amino (Fmoc)-modifier DMTr CEP
3'-Amino (Fmoc)-modifier-C3 CPG
3'-Amino (Fmoc)-modifier-C7 CPG
3'-Amino (TFA)-modifier-spacer 6 Polystyrene
3'-Amino (TFA)-modifier-spacer 7-Icaa CPG
3'-Amino (trityl)-modifier-dT CPG
3'-Amino-modifier-C6-phthalamido CPG
5'-Amino (Fmoc)-modifier-C6 CEP
5'-Amino (Fmoc)-modifier-C7 CEP
5'-Amino (MMTr)-modifiers-C6 CEP
5'-Amino (TFA)-modifier-C12 CEP
5'-Amino (TFA)-modifiers-C4 CEP
5'-Amino (TFA)-modifiers-C5 CEP
5'-Amino (TFA)-modifiers-C6 CEP
5'-Amino(MMT)-modifier-C12 CEP
Amino-modifier-13-dC CE; deoxy Cytidine N4 Amino Linker CEP
Amino-modifier-13-dC CPG
Amino-modifier-C6-dT CPG
Amino-modifier-C6-phthalamido CEP
Amino-modifiers-C6-dT CEP
DMT-free Amine C-7 Icaa CPG
ssR-Me Amino Linker
ssR-H Amino Linker
C: Spacer Modifiers and Supports
Butyl CPG (for 3’-Butanol Spacer)
DMTr-Butane-diol phosphoramidite; C4 Spacer
DMTr-Butanol polystyrene
DMTr-C3 lcaa CPG
DMTr-Dodecane-diol phosphoramidite; C12 Spacer
DMTr-Ethane-diol phosphoramidite; C2 Spacer
DMTr-Hexa-Ethyloxy-glycol phosphoramidite; Spacer 18
DMTr-Hexane-diol phosphoramidite; C6 Spacer
DMTr-Nonane-diol phosporamidite; C9 Spacer
DMTr-Propane-diol phosphoramidite; C3 Spacer
DMTr-Tetraethyloxy-glycol phosphoramidite; Spacer 12
DMTr-Triethyloxy-glycol phosphoramidite; Spacer 9
Glyceryl CPG; 1-DMTr-2-OAc-Glyceryl Succinate attached to lcaa CPG
Palmitoyl polystyrene support
Pentane diol; C5 Spacer
Polyethyleneglycol 2000 CEP
Polyethylenglycol 4500 CEP, (Avg. M.W.~4500)
Polyethylenglycol 4500 CEP, (Avg. M.W.~4500)
TOM C6 CEP
D: Thiol Modifiers and Supports
3'-Thiol-modifiers-C3-S-S CPG
5'-Thiol (trityl)-modifiers-C6 CEP, 5’-Thiol (trityl)-modifiers-C6 CPG
5'-Thiol (trityl)-modifiers-dC (N-Ac) CEP
5'-Thiol C3 SS modifier
5'-Thiol-modifiers C6 CEP; 1-Thiobenzoyl-hexanol-6 Cyanoethyl CEP
Thiol-modifiers-C6-S-S CEP
Thiol-modifiers-C6-S-S CPG; DMTr-C6-Disulfide Solid Support
Cyclic Dithiolane Disulfide Phosphoramidite, Cyclic Dithiolane Disulfide lcaa CPG
E: Branching phosphoramidites and Supports
Asymmetrical Branching CEP
Asymmetrical Branching lcaa CPG
NPPOC-DMT Asymmetrical Branching CEP
Symmetrical Branching CEP
Symmetrical Branching Polystyrene Support
Symmetrical Branching Support lcaa CPG
Labelling
A: Biotin Labelling
Biotin (BB) -TEG CEP
Biotin (BB)TM CEP
Biotin (BB)TM CPG; D-(+) Biotin lcaa CPG
Biotin dT CEP
Biotin TEG lcaa CPG
B: Psoralen labelling
Psoralen C-18 Hexaethyloxyglycol CEP
Psoralen C-2 CEP
Psoralen C-4 CEP
Psoralen C-4 lcaa CPG
Psoralen C-6 CEP
Psoralen CEP
Psoralen Support
C: Fluorescein Labelling
3'-Fluorescein-dT CPG
3'-Fluorescein-Icaa CPG
5-FAM CPG
5-Fluorescein CEP
5'-Fluorescein CPG, 6-FAM lcaa CPG (DMTr)
5'-Fluorescein-phosphoramidite; 6-FAM
5'-Hexachloro-fluorescein Phosphoramidite
5'-Tetrachloro-fluorescein Phosphoramidite
6-FAM CPG
DMTr-5-FAM Phosphoramidite
DMTr-6-FAM Phosphoramidite
Etheno-adenosine 2'/3'-lcaa CPG
Etheno-adenosine 2'-TBDMS 3'-lcaa CPG
Etheno-adenosine CEP; Etheno-2'-O-TBDMS Adenosine CEP
Etheno-cytidine 2'/3'-lcaa CPG
Etheno-cytidine 2’-TBDMS 3’-lcaa CPG
Etheno-cytidine CEP; Etheno-2'-O-TBDMS Cytidine CEP
Etheno-deoxyadenosine 3'-lcaa CPG
Etheno-deoxyadenosine CEP
Etheno-deoxycytidine 3'-lcaa CPG
Etheno-deoxycytidine CEP
Fluorescein dT CEP
Hexachloro-fluorescein Cyclohexyl CEP
Tetrachloro-fluorescein Cyclohexyl CEP
D: DNP Labelling
DNP-TEG CEP
DNP-TEG-lcaa-CPG
Naphthyl Red phosphoramidite
E: Rhodamine (Tamra) labelling
5'-TAMRA CEP
6-TAMRA Supports & Columns
Cocktail for TAMRA
N4-6-TAMRA Cytidine-3’- lcaa CPG
Rox Supports & Columns
F: Dabcyl Labelling
3'-Dabcyl CEP
3'-Dabcyl CPG
Dabcyl Succinyl diamido Polystyrene Support
Dabsyl-dT CEP
G: Cholesterol
Cholesterol (Plant) TEG CEP
Cholesterol (TEG) CEP (Will Generate DMT color)
Cholesterol C4 lcaa CPG
Cholesterol TEG lcaa CPG
TEG Cholesterol (Tetra Ethylene Glycol) CEP
TEG Cholesterol (Tetra Ethylene Glycol) Phosphonate
TEG Cholesterol (Tetra Ethylene Glycol) -H- Phosphonate TEA Salt"
H: Tocopherol
(+)-a-Tocopherol-Phosphoramidite
I: Carboxyl Generating reagents
5'-Carboxyl Group Generating Reagent; Thymidine-Succinyl Hexamide CEP
C-5 Carboxy Introducing Amidite; Deoxy Uridine 5-Methylacrylate CEP
Hexane-6-Carboxyamido (p-benzaldehyde)- 1-ol CEP
Undecanoic CEP
J: Puromycin Labelling
3'-L-(O-Methyl)-Tyrosine-puromycin-2'- Icaa CPG
Puromycin-2'-Succinyl Icaa CPG
A: Chemical Phosphorylation and Supports
5'- Phosphorylation; Bis-cyanoethyl-N,N-diisopropyl CEP
3'-Phosphate-ON Icaa CPG
5-Phosphorylation (generates DMTr color)-(5-Phosphate-ON Reagent)
B: Amino Modifiers and Supports
3'-Amino (Fmoc)-modifier DMTr CEP
3'-Amino (Fmoc)-modifier-C3 CPG
3'-Amino (Fmoc)-modifier-C7 CPG
3'-Amino (TFA)-modifier-spacer 6 Polystyrene
3'-Amino (TFA)-modifier-spacer 7-Icaa CPG