The synthesis of RNA is conventionally more sensitive than DNA. This poses challenges in developing very similar synthesis compounds such as amidites and supports, but with the right chemistry to counter RNA-specific hurdles. Stability and structural confirmation are routine offenders, and both can be countered by utilizing backbone phosphorothioation and reverse synthesis techniques. β-L-RNA-Spiegelmers and minor RNA phosphoramidites offer novel solutions to end-user specificity. While TOM-amidites are highly suitable for large scale therapeutic grade RNA synthesis, long RNA sequences, aptamers, and biologically significant RNA molecules.



See the full list of in-house RNA Compounds used in our synthesis below:





RNA Synthesis
A: CE Phosphoramidites: TBDMS Protected 
(1)-TBDMS Protected 
Adenosine (N-Ac)-DMTr-3'-CEP
Adenosine (N-Bz)-DMTr-3'-CEP
Adenosine (N-Bz)-MMTr-3'-CEP
Cytidine (N-Ac)-DMTr-3'-CEP
Cytidine (N-Bz)-DMTr-3'-CEP
Cytidine (N-Bz)-MMTr-3'-CEP
Guanosine (N-Ac)-DMTr-3'-CEP
Guanosine (N-DMF)-DMTr-3'-CEP
Guanosine (N-iBu)-DMTr-3'-CEP
Guanosine (N-iBu)-MMTr-3'-CEP
Uridine-DMTr-3'-CEP
Uridine-MMTr-3'-CEP
(2)-TBDMS Protected (Ultramild  base protection) 
Adenosine (N-PAC)-DMTr-3'-CEP
Adenosine (N-PAC)-MMTr-3'-CEP
Cytidine (N-Ac)-DMTr-3'-CEP
Cytidine (N-PAC)-DMTr-3'-CEP
Cytidine (N-tBPAC)-MMTr-3'-CEP
Guanosine (N-iprPAC)-DMTr-3'-CEP
Guanosine (N-PAC)-DMTr-3'-CEP
Guanosine (N-PAC)-MMTr-3'-CEP
Guanosine (N-Phenyl-Ac)-DMTr-3'-CEP
(3)-TOM Protected 
2'-TOM-Adenosine (N-Ac)-DMTr-3'-CEP
2'-TOM-Cytidine (N-Ac)-DMTr-3'-CEP
2'-TOM-Guanosine (N-Ac)-DMTr-3'-CEP
2'-TOM-Uridine-DMTr-3'-CEP
(4)-Acetal levulinyl ester Protected 
2'-ALE-Adenosine (N-PAc)-5'-NPPOC-3'-CEP
2'-ALE-Cytidine (N-iBu)-5'-NPPOC-3'-CEP
2'-ALE-Guanosine (N-PAc)-5'-NPPOC-3'-CEP
2'-ALE-Uridine-5'-NPPOC-3'-CEP
(5)-Pivaloyloxymethyl Protected 
2'-Pivom-Adenosine (N-PAc)-DMTr-3'-CEP
2'-Pivom-Cytidine (N-Ac)-DMTr-3'-CEP
2'-Pivom-Guanosine (N-iPrPAC)-DMTr-3'-CEP
2'-Pivom-Uridine-DMTr-3'-CEP
B: Supports 
(1)-TBDMS Protected 3'-CPG 
Adenosine (N-Bz)-DMTr-3'-Icaa CPG
Cytidine (N-Ac)-DMTr-3'-Icaa CPG
Cytidine (N-Bz)-DMTr-3'-Icaa CPG
Guanosine (N-iBu)-DMTr-3'-Icaa CPG
Uridine-DMTr-3'-Icaa CPG
(2)-TBDMS protected 3'-CPG (Ultramild  base protection) 
Adenosine (N-PAC)-DMTr-3'-Icaa CPG
Adenosine (N-tBPAC)-DMTr- 3'-Icaa CPG
Cytidine (N-PAC)-DMTr-3'-Icaa CPG
Guanosine (N-PAC)-DMTr-3'-Icaa CPG
Guanosine (N-tBPAC)-DMTr-3'-Icaa CPG
Uridine-DMTr-3'-Icaa CPG
(3)-TBDMS protected 3'-Oxalyl CPG 
Adenosine (N-PAC)-DMTr-3'-oxalyl Icaa-CPG
Ammonia Free Oligo Deprotection Solution
Cytidine (N-PAC)-DMTr-3'-oxalyl  Icaa CPG
Guanosine (N-iBu)-DMTr-3'-oxalyl Icaa CPG
Guanosine (N-PAC)-DMTr-3'-oxalyl Icaa CPG
Uridine-DMTr-3'-oxalyl Icaa CPG
(4)-TBDMS protected 3'-Polystyrene 
Adenosine (N-Bz)-DMTr-3'-succinyl Polystyrene
Cytidine (N-Ac)-DMTr-3'-succinyl Polystyrene
Cytidine (N-Bz)-DMTr-3'-succinyl Polystyrene
Guanosine (N-iBu)-DMTr-3'-succinyl Polystyrene
Uridine-DMTr-3'-succinyl Polystyrene
(5)-TBDMS protected-3'-Non-clevable long chain spacer 
2'-TBDMS Guanosine (N-iBu)-DMTr-3'-non-clevable long chain space
(6)-TOM protected 3'-CPG 
2'-TOM-Adenosine (N-Ac)-DMTr-3'-Icaa CPG
2'-TOM-Cytidine (N-Ac)-DMTr-3'-Icaa CPG
2'-TOM-Guanosine (N-Ac)-DMTr-3'-Icaa CPG
2'-TOM-Uridine-DMTr-3'-Icaa CPG
B: Backbone modification:Methyl Phosphoramidite 
P-O-Methyl-adenosine (N-Bz)-DMTr-3'-CEP
P-O-Methyl-adenosine (N-Bz)-MMTr-3'-CEP
P-O-Methyl-adenosine (N-PAC)-DMTr-3'-CEP
P-O-Methyl-adenosine (N-PAC)-MMTr-3'-CEP
P-O-Methyl-cytidine (N-Bz)-DMTr-3'-CEP
P-O-Methyl-cytidine (N-PAC)-DMTr-3'-CEP
P-O-Methyl-guanosine (N-iBu)-DMTr-3'-CEP
P-O-Methyl-guanosine (N-iBu)-MMTr-3'-CEP
P-O-Methyl-guanosine (N-PAC)-DMTr-3'-CEP
P-O-Methyl-guanosine (N-PAC)-MMTr-3'-CEP
P-O-Methyl-uridine-DMTr-3'-CEP
RNA:5'→ 3' Synthesis
A: CE Phosphoramidites: TBDMS Protected 
Adenosine (N-Bz)-DMTr-5'-CEP
Cytidine (N-Ac)-DMTr-5'-CEP
Cytidine (N-Bz)-DMTr-5'-CEP
Guanosine (N-iBu)-DMTr-5'-CEP
Guanosine (N-iprPAC)-DMTr-5'-CEP
Uridine-DMTr-5'-CEP
B: Supports-TBDMS protected 5'-CPG 
Adenosine (N-Bz)-DMTr-5'-Icaa CPG
Cytidine (N-Ac)-DMTr-5'-Icaa CPG
Cytidine (N-Bz)-DMTr-5'-Icaa CPG
Guanosine (N-iBu)-DMTr-5'-Icaa CPG
Uridine-DMTr-5'-Icaa CPG
Minor RNA Bases
A: Minor RNA Phosphoramidites
(1)-TBDMS Protected RNA Phosphoramidites
2,2'-Anhydro-uridine-DMTr-3'-CEP
2-Amino-adenosine-DMTr-3'-CEP, (2,6-Diamino Purine DMTr-3'-CEP)
2-Amino-purine (N-DMF)-DMTr-3'-CEP
2-Amino-purine (N-iBu)-DMTr-3'-CEP
2-One-pyridine-DMTr-3'-CEP; Zebularine CEP
4-Triazolyl-uridine-DMTr-3'-CEP
4-Thio-uridine-DMTr-3'-CEP
2-Thio-uridine-DMTr-3'-CEP
5-Bromo-cytidine (N-Bz)-DMTr-3'-CEP
5-Bromo-uridine-DMTr-3'-CEP
5-Fluoro-2'-deoxyuridine-DMTr-3'-CEP
5-Iodo-cytidine (N-Bz)-DMTr-3'-CEP
5-Iodo-uridine-DMTr-3'-CEP
5-Methyl-cytidine (N-Bz)-DMTr-3'-CEP
8-Bromo-adenosine (N-DBF)-DMTr-3'-CEP
8-Bromo-adenosine (N-DMF)-DMTr-3'-CEP
8-Bromo-guanosine (N-DMF)-DMTr-3'-CEP
8-methyl-guanosine (N-iBu)-DMTr-3'-CEP
8-Oxo-7-allyl-guanosine (N-iBu)-DMTr-3'-CEP
8-Oxo-guanosine (N-DMF)-DMTr-3'-CEP
Inosine-DMTr-3'-CEP
N3-Methyl-thymidine-DMTr-3'-CEP
N3-Methyl-uridine-DMTr-3'-CEP
N3-Thiobenzoylethyl-uridine-DMTr-3'-CEP
N6N6-Dimethyl-adenosine-5'-DMTr-3'-CEP
N6-Benzyl-adenosine-DMTr-3'-CEP
N6-isopentenyl-adenosine-DMTr-3'-CEP
N4-Ethyl-cytidine-DMTr-3'-CEP
Purine-riboside-DMTr-3'-CEP; Nebularine CEP
Thymidine-DMTr-3'-CEP
5,6-Dihydro-uridine-DMTr-3'-CEP
7-Deaza-7-cyano-adenosine (N-Bz)-DMTr-3'-CEP
7-Deaza-adenosine (N-Bz)-DMTr-3'-CEP; Tubercidin
7-Deaza-guanosine (N-Ac)-DMTr-3'-CEP
7-Deaza-guanosine (N-iBu)-DMTr-3'-CEP
7-Deaza-guanosine (N-iBu)-DMTr-5'-CEP
Iso-cytidine (N-DBF)-DMTr-3'-CEP
Iso-guanosine (N-DBF)-DMTr-3'-CEP
Adenosine (N-Bz)-DMTr-2'-CEP
Cytidine (N-Ac)-DMTr-2'-CEP
Cytidine (N-Bz)-DMTr-2'-CEP
Guanosine (N-iBu)-DMTr-2'-CEP
Inosine-DMTr-2'-CEP
Uridine-DMTr-2'-CEP
P-O-Ethyl-adenosine (N-PAC)-DMTr-3'-CEP
P-O-Ethyl-cytidine (N-PAC)-DMTr-3'-CEP
P-O-Ethyl-guanosine (N-PAC)-DMTr-3'-CEP
P-O-Ethyl-uridine-DMTr-3'-CEP
Pseudo-uridine-DMTr-3'-CEP
β-L-RNA-Spiegelmers
β-L-Adenosine (N-Bz)-DMTr-3'-CEP
β-L-Cytidine (N-Ac)-DMTr-3'-CEP
β-L-Cytidine (N-Bz)-DMTr-3'-CEP
β-L-Guanosine (N-iBu)-DMTr-3'-CEP
β-L-Thymidine-DMTr-3'-CEP
β-L-Uridine-DMTr-3'-CEP
(2)Amino TFA Protected RNA Phosphoramidites
2'-Amino(TFA)-cytidine (N-Bz)-DMTr-3'-CEP
2-Amino-2'-Amino (TFA)-uridine-DMTr-3'-CEP
5'-Amino (TFA)-cytidine (N-Ac)-3'-CEP
(3)-Acetyl Protected RNA Phosphoramidites
2',3'-Diacetyl-guanosine (N-iBu)-5'-CEP
2',3'-Diacetyl-uridine-5'-CEP
B: Supports-Minor-RNA-TBDMS Protected 3'-CPG
(1)-TBDMS Protected RNA 3'-CPG
2,2'-Anhydro-uridine-DMTr-3'-Icaa CPG
2-Amino-adenosine-DMTr-3'-Icaa CPG
2-Amino-purine-DMTr-3'-Icaa CPG
2-One-pyridine-DMTr-3'-Icaa CPG; Zebularine CPG
5-Bromo-cytidine (N-Bz)-DMTr-3'-Icaa CPG
5-Bromo-uridine-DMTr-3'-Icaa CPG
5-Fluoro-uridine-DMTr-3'-Icaa CPG
5-Iodo-cytidine (N-Bz)-DMTr-3'-Icaa CPG
5-Iodo-uridine-DMTr-3'-Icaa CPG
5-Methyl-cytidine (N-Bz)-DMTr-3'-Icaa CPG
7-Deaza-adenosine (N-Bz)-DMTr-3'-Icaa CPG; Tubercidin CPG
7-Deaza-guanosine (N-iBu)-DMTr-3'-Icaa CPG
8-Bromo-adenosine (N-DBF)-DMTr-3'-Icaa CPG
8-Methyl-guanosine (N-iBu)-DMTr-3'-Icaa CPG
Adenosine (N-Ac)-DMTr-3'-Icaa CPG
Adenosine (N-Bz)-DMTr-3'-Icaa CPG
Cytidine (N-Ac)-DMTr-3'-Icaa CPG
Cytidine (N-Bz)-DMTr-3'-Icaa CPG
Guanosine (N-iBu)-DMTr-3'-Icaa CPG
Inosine-DMTr-3'-Icaa CPG
Iso-Cytidine  (N-DBF)-DMTr-3'-Icaa CPG
Iso-Guanosine (N-DBF)-DMTr-3'-Icaa CPG
N3-Methyl-thymidine-DMTr-3'-Icaa CPG